Simultaneous Determination and Validation of Paracetamol and Codeine Phosphate in Pharmaceutical Preparation by Rp-hplc

An accurate, simple, reproducible and sensitive method for the determination of Paracetamol and Codeine phosphate was developed and validated. Paracetamol and Codeine were separated by HPLC using a LiChrospher® RP-18 column and isocratic elution with a flow rate of 1.0 ml/min. Mixture of Acetonitile with Buffer solution (pH=2.5) (15:85) was used as mobile phase. The detection was at 210 nm wavelength. The proposed method was validated for linearity, accuracy, precision, limit of detection and limit of quantification. The linear range of determination for Paracetamol and Codeine phosphate were 100-1000μg/ml and 6-60 μg/ml, respectively. The percentage recovery of Paracetamol and Codeine phosphate were obtained form range of 99.88-100.2% and 99.33-100.3% respectively. The developed method is suitable for routine quality control analysis of titled drugs in combination of tablet formulation.